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bacterial 16s ribosomal rna gene targeted sequencing  (Zymo Research)


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    Zymo Research bacterial 16s ribosomal rna gene targeted sequencing
    Bacterial 16s Ribosomal Rna Gene Targeted Sequencing, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/16S/pmc12800435-178-9-25
    Average 99 stars, based on 1176 article reviews
    bacterial 16s ribosomal rna gene targeted sequencing - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Biodegradation and detoxification of malachite green dye by crude manganese peroxidase produced by Pseudomonas aeruginosa MF14446.1 and Enterobacter roggenkampii CP033800.1.
    Article Snippet: Genomic DNA was extracted from the cultures using the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Catalogue No. D6005). .. The 16S target was amplified using OneTaq QuickLoad 2X Master Mix (NEB, Catalogue No. M0486) with primers (16S-27F, AR TIC LE IN PR ES S 16S rDNA sequence target using 5` to 3` sequence AGAGTTTGATCMTGGCTCAG) and 16S-1492R, 16S rDNA The PCR products were run on a gel, and the gel was extracted with the Zymoclean Gel Recovery Kit (Zymo Research, Catalogue No. D4001). .. The extracted fragments were sequenced in the forward and reverse direction (Nimagen, Brilliant Dye Terminator Cycle Sequencing Kit V3.1, BRD3-100/1000) and purified (Zymo Research, ZR-96 DNA Sequencing Clean-up Kit, Catalogue No. D4050).

    Sequencing:

    Article Title: Biodegradation and detoxification of malachite green dye by crude manganese peroxidase produced by Pseudomonas aeruginosa MF14446.1 and Enterobacter roggenkampii CP033800.1.
    Article Snippet: Genomic DNA was extracted from the cultures using the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Catalogue No. D6005). .. The 16S target was amplified using OneTaq QuickLoad 2X Master Mix (NEB, Catalogue No. M0486) with primers (16S-27F, AR TIC LE IN PR ES S 16S rDNA sequence target using 5` to 3` sequence AGAGTTTGATCMTGGCTCAG) and 16S-1492R, 16S rDNA The PCR products were run on a gel, and the gel was extracted with the Zymoclean Gel Recovery Kit (Zymo Research, Catalogue No. D4001). .. The extracted fragments were sequenced in the forward and reverse direction (Nimagen, Brilliant Dye Terminator Cycle Sequencing Kit V3.1, BRD3-100/1000) and purified (Zymo Research, ZR-96 DNA Sequencing Clean-up Kit, Catalogue No. D4050).

    Polymerase Chain Reaction:

    Article Title: Biodegradation and detoxification of malachite green dye by crude manganese peroxidase produced by Pseudomonas aeruginosa MF14446.1 and Enterobacter roggenkampii CP033800.1.
    Article Snippet: Genomic DNA was extracted from the cultures using the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Catalogue No. D6005). .. The 16S target was amplified using OneTaq QuickLoad 2X Master Mix (NEB, Catalogue No. M0486) with primers (16S-27F, AR TIC LE IN PR ES S 16S rDNA sequence target using 5` to 3` sequence AGAGTTTGATCMTGGCTCAG) and 16S-1492R, 16S rDNA The PCR products were run on a gel, and the gel was extracted with the Zymoclean Gel Recovery Kit (Zymo Research, Catalogue No. D4001). .. The extracted fragments were sequenced in the forward and reverse direction (Nimagen, Brilliant Dye Terminator Cycle Sequencing Kit V3.1, BRD3-100/1000) and purified (Zymo Research, ZR-96 DNA Sequencing Clean-up Kit, Catalogue No. D4050).



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    bacterial 16s ribosomal rna gene targeted sequencing  (Zymo Research)
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    Zymo Research bacterial 16s ribosomal rna gene targeted sequencing
    <t>Cherp</t> serving an effective treatment target for IS (A) The neuron counts of cluster 3 and 5. (B) The Cherp expression in each cluster. (C) The WB image of Cherp expression after OGD/R process. (D) The IF images of Cherp expression after OGD/R process. Scale bars = 100 μm. (E) The efficiency validation of Cherp knocking down using <t>shRNA.</t> (F) The cell viability assay after Cherp knocking down. (G) LDH leakage assay after Cherp knocking down. (H) TUNEL assay after Cherp knocking down. Scale bars = 100 μm. (I) WB image of apoptosis-related markers after Cherp knocking down. (J) Morphology of cerebral edema in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery. (K) TTC staining of cerebral infarction in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery (Dashed area represents the infarcted brain region). (L) Statistics analysis of brain water content ( n = 5 mice/group). (M) Quantification of infarct area ( n = 5 mice/group). (N) The functional enrichment of Cherp high cells related pathways. (O) The intracellular calcium imaging after Cherp knocking down. Scale bars = 100 μm (P) Quantitative analysis of intracellular Ca 2+ fluorescence intensity in primary neurons under indicated conditions ( n = 3 independent experiments). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 as determined by Student’s t test.
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    Image Search Results


    Input and Output of each stage

    Journal: Current Protocols

    Article Title: A Guide to Enzymatic Targeted Methylation Sequencing Bioinformatics Workflow: Best Practices

    doi: 10.1002/cpz1.70464

    Figure Lengend Snippet: Input and Output of each stage

    Article Snippet: Targeted methylation profiling was performed using the NEBNext EM‐seq Kit combined with the Twist Targeted Methylation Sequencing platform (Twist Bioscience, USA), following the manufacturer's instructions (New England Biolabs, ; “Twist Targeted Methylation Sequencing Protocol | Twist Bioscience,” accessed July 30, 2025).

    Techniques: Sequencing, Methylation, Analysis, Control, Tiling

    Estimated duration of the complete analysis

    Journal: Current Protocols

    Article Title: A Guide to Enzymatic Targeted Methylation Sequencing Bioinformatics Workflow: Best Practices

    doi: 10.1002/cpz1.70464

    Figure Lengend Snippet: Estimated duration of the complete analysis

    Article Snippet: Targeted methylation profiling was performed using the NEBNext EM‐seq Kit combined with the Twist Targeted Methylation Sequencing platform (Twist Bioscience, USA), following the manufacturer's instructions (New England Biolabs, ; “Twist Targeted Methylation Sequencing Protocol | Twist Bioscience,” accessed July 30, 2025).

    Techniques: Analysis, QC/EQA/Proficiency, Sequencing, Methylation, Tiling

    Cherp serving an effective treatment target for IS (A) The neuron counts of cluster 3 and 5. (B) The Cherp expression in each cluster. (C) The WB image of Cherp expression after OGD/R process. (D) The IF images of Cherp expression after OGD/R process. Scale bars = 100 μm. (E) The efficiency validation of Cherp knocking down using shRNA. (F) The cell viability assay after Cherp knocking down. (G) LDH leakage assay after Cherp knocking down. (H) TUNEL assay after Cherp knocking down. Scale bars = 100 μm. (I) WB image of apoptosis-related markers after Cherp knocking down. (J) Morphology of cerebral edema in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery. (K) TTC staining of cerebral infarction in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery (Dashed area represents the infarcted brain region). (L) Statistics analysis of brain water content ( n = 5 mice/group). (M) Quantification of infarct area ( n = 5 mice/group). (N) The functional enrichment of Cherp high cells related pathways. (O) The intracellular calcium imaging after Cherp knocking down. Scale bars = 100 μm (P) Quantitative analysis of intracellular Ca 2+ fluorescence intensity in primary neurons under indicated conditions ( n = 3 independent experiments). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 as determined by Student’s t test.

    Journal: iScience

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke

    doi: 10.1016/j.isci.2026.116257

    Figure Lengend Snippet: Cherp serving an effective treatment target for IS (A) The neuron counts of cluster 3 and 5. (B) The Cherp expression in each cluster. (C) The WB image of Cherp expression after OGD/R process. (D) The IF images of Cherp expression after OGD/R process. Scale bars = 100 μm. (E) The efficiency validation of Cherp knocking down using shRNA. (F) The cell viability assay after Cherp knocking down. (G) LDH leakage assay after Cherp knocking down. (H) TUNEL assay after Cherp knocking down. Scale bars = 100 μm. (I) WB image of apoptosis-related markers after Cherp knocking down. (J) Morphology of cerebral edema in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery. (K) TTC staining of cerebral infarction in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery (Dashed area represents the infarcted brain region). (L) Statistics analysis of brain water content ( n = 5 mice/group). (M) Quantification of infarct area ( n = 5 mice/group). (N) The functional enrichment of Cherp high cells related pathways. (O) The intracellular calcium imaging after Cherp knocking down. Scale bars = 100 μm (P) Quantitative analysis of intracellular Ca 2+ fluorescence intensity in primary neurons under indicated conditions ( n = 3 independent experiments). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 as determined by Student’s t test.

    Article Snippet: The shRNA sequences targeting Cherp were generated at Sangon Biotech.

    Techniques: Expressing, Biomarker Discovery, shRNA, Viability Assay, TUNEL Assay, Staining, Functional Assay, Imaging, Fluorescence